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| Registros recuperados: 67 | |
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| Le Guyader, Soizick; Le Saux, Jean-claude; Ambert-balay, Katia; Krol, Joanna; Serais, Ophelie; Parnaudeau, Sylvain; Giraudon, Helene; Delmas, Gilles; Pommepuy, Monique; Pothier, Pierre; Atmar, Robert L.. |
| Following a flooding event close to a shellfish production lagoon, 205 cases of gastroenteritis were linked to oyster consumption. Twelve stool samples from different individuals were collected. Analysis showed that eight samples were positive for multiple enteric viruses, and one stool sample had seven different enteric viruses. Analysis of shellfish implicated in the outbreak allowed detection of the same diversity of enteric viruses, with some viral genomic sequences being identical to those obtained from stool sample analysis. Shellfish were contaminated by as many as five different enteric viruses. For the first time in Europe, Aichi virus was identified in oyster samples. Shellfish samples collected over 3 weeks following the outbreak showed a... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6099.pdf |
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| Guillois-becel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A. M.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, V.. |
| Following the notification of nine hepatitis A cases clustered in the Cotes d'Armor district in northwestern France, epidemiological, environmental and microbiological investigations were set up in order to identify the source and vehicle of contamination and implement control measures. In total, 111 cases were identified in the outbreak, all of whom lived or had stayed as tourists in the Cotes d'Armor district. Of the cases, 87% had eaten raw shellfish, and 81% specifically oysters. Traceback investigations carried out on raw shellfish consumed by the cases showed that the raw shellfish originated from a single shellfish farm. The shellfish were probably contaminated either in the submersible tanks or in a depuration land-based tank where they were... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6685.pdf |
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| Zakhour, Maha; Maalouf, Haifa; Di Bartolo, Ilaria; Haugarreau, Larissa; Le Guyader, Francoise; Ruvoen-clouet, Nathalie; Le Saux, Jean-claude; Ruggeri, Franco Maria; Pommepuy, Monique; Le Pendu, Jacques. |
| Noroviruses (NoV) are major agents of acute gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Previous studies showed that some human strains bind to oyster tissues through carbohydrate ligands that are similar to their human receptors. Thus, based on presentation of shared norovirus carbohydrate ligands, oysters could selectively concentrate animal strains with increased ability to overcome species barriers. In comparison with human GI and GII strains, bovine GIII NoV strains, although frequently detected in bovine feces and waters of two estuaries of Brittany, were seldom detected in oysters grown in these estuaries. Characterization of the carbohydrate ligand from a new GIII strain indicated recognition of the... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12534/9527.pdf |
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| Pommepuy, Monique. |
| The study 'admission capacity of the marine environment" was conducted by IFREMER 1980 to 1983 in the Brest Roadstead at the request of the Ministry of Environment. This study was financed jointly by the Board of Health and IFREMER. The problem was the study of the fate of bacterial discharges in a sea subjected to tides. The selected approach consisted to simulate discharges from the principal pollution sources and to calculate the theoretical dilution of fecal bacteria in the Northern zone of the Roadstead. Spreading coefficients were determined by "on the site" measurements of parameters which may be considered as conservative in defined periods (e.g. ammonium in winter). Results show that in the conditions of the styay, the disappearance of fecal... |
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| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00054/16572/14067.pdf |
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| Miossec, Laurence; Le Guyader, Francoise; Haeghebaert, S.; Gasnier, Ph.; Bellier, J.y.; Vaillant, V.; Camus, P.; Pommepuy, Monique; Abou-saleh, M.j.; Clavelin, Ph.; Bobo, J.p.; Masson, D.; Desenclos, J.c.. |
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| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00001/11244/7780.pdf |
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| Miossec, Laurence; Besseau, Laetitia; Haugarreau, Larissa; Le Guyader, Francoise; Menard, Dominique; Pommepuy, Monique; Abadie, Eric; Comps, Marie-annick; Guillou, Jean-louis; Le Bec, Claude; Sauvagnargues, Jean-claude; Bon, Fabienne; Jamot, B.; Kohli E, Evelyne; Potier, P.; Daurat, G.; Guyonnet, J.p.. |
| Les microorganismes, bactéries et virus d'origine entérique, sont abondants dans les rejets urbains. Si la charge bactériologique contenue dans les eaux usées est notablement réduite lors des traitements appliqués dans les stations d'épurations, de nombreux microorganismes persistent et sont rejetés avec les eaux épurées dans le milieu récepteur, en rivière ou directement en mer. Le développement de méthodes analytiques basées sur la biologie moléculaire a permis récemment la détection de virus entériques non cultivables dans les échantillons de l'environnement, tels que les entérovirus, le virus de l'hépatite A, les calicivirus, les astrovirus et les rotavirus. En France, les données publiées sur la présence de ces virus entériques, détectés par RTPCR,... |
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| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00044/15552/12941.pdf |
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| Pommepuy, Monique; Caprais, Marie-paule; Le Mennec, Cecile; Parnaudeau, Sylvain; Le Guyader, Francoise; Camus, Patrick; Dimeet, Joel; Treguier, Cathy; Bon, Fabienne; Pothier, Pierre; Kohli E, Evelyne; Brachet, R.; Flahault, A.; Sarrette, B.; Vilagines, P.. |
| Le développement des activités humaines dans les régions littorales entraîne le rejet de germes pathogènes d'origine fécale dans l'environnement estuarien et marin. L'amélioration des traitements d'épuration, mais aussi la mise en place de normes bactériologiques pour surveiller les zones de production conchylicole, ont entraîné une diminution des épidémies liées à la consommation de coquillages. Ainsi, à l'heure actuelle, même si l'origine de ces gastro-entérites n'est pas élucidée dans sa globalité (rôle probable des vibrios ou d'autres bactéries), les virus semblent jouer un rôle important et leur implication té clairement établie dans les phénomènes épidémiques (Miossec et Vaillant, 2001) Les principaux virus humains susceptibles d'être détectés dans... |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00044/15549/12936.pdf |
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| Pommepuy, Monique; Le Guyader, F.s; Le Pendu, Jacques; Poncet, D; Thebault, Anne; Le Saux, Jean-claude. |
| L'objectif général du projet est de comprendre le rôle du milieu marin, et en particulier des coquillages, sur la sélection des souches de norovirus humaines ou animales et sur leur pathogénicité potentielle pour l'homme. Des évènements récents ont montré l 'importance du potentiel zoonotique des virus, par voie directe, mais aussi au travers d'aliments potentiellement infectés ou contaminés [Yazaki et al., 2003] 1; [Stavrinides et Guttman, 2004]. L'analyse de l'évolution des Caliciviridae montre ainsi que le virus de l'exanthème vésiculeux du porc (VESV) serait apparu via l'alimentation contenant des farines de poissons jnfectés [Etherington et al. , 2006]. L'émergence de pathogènes au travers d'un passage inter - espèce correspond à des évènements rares,... |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00044/15550/12937.pdf |
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| Le Guyader, Soizick; Parnaudeau, Sylvain; Schaeffer, Julien; Bosch, Albert; Loisy, Fabienne; Pommepuy, Monique; Atmar, Robert L.. |
| Noroviruses (NoVs) are the most common viral agents of acute gastroenteritis in humans, and high concentrations of NoVs are discharged into the environment. As these viruses are very resistant to inactivation, the sanitary consequences are contamination of food, including molluscan shellfish. There are four major problems with NoV detection in shellfish samples: low levels of virus contamination, the difficulty of efficient virus extraction, the presence of interfering substances that inhibit molecular detection, and NoV genetic variability. The aims of this study were to adapt a kit for use with a method previously shown to be efficient for detection of NoV in shellfish and to use a one step real-time reverse transcription-PCR method with addition of an... |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6137.pdf |
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| Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique. |
| The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when... |
| Tipo: Text |
Palavras-chave: Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus. |
| Ano: 2002 |
URL: https://archimer.ifremer.fr/doc/2002/publication-1269.pdf |
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| Le Guyader, Soizick; Bon, Fabienne; Demedici, Dario; Parnaudeau, Sylvain; Bertone, Alessandra; Crudeli, Silvia; Doyle, Aoife; Zidane, Mohamed; Suffredini, Elisabetta; Kohli E, Evelyne; Maddalo, Francesco; Monini, Marina; Gallay, Anne; Pommepuy, Monique; Pothier, Pierre; Ruggeri, Franco M.. |
| An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to... |
| Tipo: Text |
Palavras-chave: Seafood; Contamination; Norovirus; Epidemiology; Virology; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2096.pdf |
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| Le Saux, Jean-claude; Derolez, Valerie; Brest, G.; Le Guyader, Soizick; Pommepuy, Monique. |
| For shellfish, the viral contamination clearly occurs in the first step of the process i.e. in growing and harvesting areas. As opposed to other foods, there is no proof that other routes (foodhandlers, aerosol), could be at the origin of the seafood contamination (Koopmans & Duizer, 2004). Most of the time, untreated sewage are at the origin of the presence of viruses in shellfish. However, direct contamination by ill people working in the growing area, has also been reported (Berg et al., 2000; Butt et al, 2004). |
| Tipo: Text |
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| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00066/17733/15254.pdf |
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| Pommepuy, Monique. |
| Le devenir des bactéries entériques rejetées en mer a été étudié sur cinq zones côtières du littoral français : la côte Ouest du Cotentin, la rade de Brest, l'estuaire de Morlaix, la rade de Toulon et la Baie de Fort de France. Sur chaque site, une étude a permis de déterminer les concentrations en coli formes thermotolérants et en streptocoques fécaux dans les principaux apports. Les salmonelles ont également été recherchées sur trois de ces secteurs. Un calcul de flux permet d'apprécier l'importance des rejets urbains. Dans ces eaux généralement très chargées en contamination fécale, la présence de salmonelles a presque toujours été détectée. La qualité des eaux littorales et des sédiments côtiers a ensuite fait l'objet d'observations. Les... |
| Tipo: Text |
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| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00052/16319/13822.pdf |
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| Gantzer, Christophe; Dubois, Éric; Crance, Jean-marc; Billaudel, Sylviane; Kopecka, Helena; Schwartzbrod, Louis; Pommepuy, Monique; Le Guyader, Françoise. |
| To improve the knowledge of the survival of enteric viruses in a marine environment, the influence of physico-chemical parameters (temperature, UV, salinity) on the survival of infectious poliovirus 1 and hepatitis A Virus (HAV) in seawater was first studied, the influence of suspended solids (SS) on poliovirus adsorption and survival in seawater was then evaluated and the detection of rotavirus genome in environmental samples (shellfish, river water, treated wastewater) was finally investigated. The results show that temperature has a major impact on virus survival in seawater as the time necessary to inactivate 90 % of the virus (T-90) is 671 days at 4 degrees C and only 25 days at 25 degrees C. Ultraviolet light (42 mW s cm(-2)) rapidly inactivates... |
| Tipo: Text |
Palavras-chave: Environmental factors; Survival; Seawater; Enteric viruses; Facteurs environnementaux; Survie; Eau de mer; Virus entériques. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-846.pdf |
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| Mieszkin, Sophie; Furet, Jean-pierre; Corthier, Gerard; Pommepuy, Monique; Le Saux, Jean-claude; Bougeard, Morgane; Hervio Heath, Dominique; Gourmelon, Michele. |
| The microbiological quality of coastal waters and shellfish harvesting areas in Brittany (France) can be affected by faecal pollutions from human activities and animal breeding (especially pigs and cattle). To discriminate among faecal pollution of human and animal origin, a library-independent microbial source tracking method was selected: Bacteroidales host-specific 16S rRNA gene markers by real-time Polymerase Chain Reaction (PCR). A human-specific Bacteroidales marker (Hum-1-Bac) was designed. Tested on faecal samples, the Hum-1-Bac marker showed 95 % sensitivity and 95 % specificity (n= 80). Average values (± STD) of the Hum-1-Bac marker were found to be 7.3 ± 1.4 16S rRNA gene copies per g wet faeces in human faeces samples (n=10) and 5.7 ± 1.3 log10... |
| Tipo: Text |
Palavras-chave: Microbial Source Tracking; Host-Specific Bacteroidales Marker; 16S rRNA Gene; Real-Time PCR; Faecal Contamination. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00021/13259/10306.pdf |
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| Alzieu, Claude; Abarnou, Alain; Bassoullet, Philippe; Boutier, Bernard; Chiffoleau, Jean-francois; Crenn, Isabelle; Derrien, Annick; Erard-le Denn, Evelyne; Gourmelon, Michele; Guiliaud, Jean-françois; Silva Jacinto, Ricardo; Le Cann, Pierre; Le Guyader, Françoise; Le Hir, Pierre; L'Yavanc, Jacky; Mauvais, Jean-louis; Menard, Dominique; Michel, Pierre; Monbet, Yves; Pommepuy, Monique; Quiniou, Francoise. |
| Port activities require continuous or occasional dredging of considerable quantities of sediments : approximately 50 million m3 along the French coastline. A large proportion of the dredgings is authorized for dumping at sea in geographically delimited areas and following an impact evaluation of their potential harmfulness to the marine environment. The environmental risks assessment linked to dredging operations relies on knowledge related to several scientific disciplines contributing to changes in the regulations. This work, intended both for managers and for environmental scientists and engineers, is designed to take stock of the current state-of-the-art in the field of sediment contamination and related ecotoxicological hazards, along with a review of... |
| Tipo: Text |
Palavras-chave: Regulations; Management; Impact; Contaminants; Dredging; Réglementation; Gestion; Impact; Contaminants; Dragages. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-1040.pdf |
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| Maalouf, Haifa; Pommepuy, Monique; Le Guyader, Soizick. |
| Human fecal wastes contain a large variety of viruses that can enter the environment through discharge of waste materials from infected individuals. Despite the high diversity of viruses that are introduced into the environment by human fecal pollution, only a few have been recognized to cause disease in association with consumption of contaminated shellfish. To explain bivalve mollusks contamination, several factors including human epidemiology, virus persistence through sewage treatment plant, and shellfish uptake may be suggested. Considering different outbreaks described in the literature, the most common route for transmission is accidental contamination after heavy rainfall, when extra loads cause an overflow, and release of untreated sewage into the... |
| Tipo: Text |
Palavras-chave: Shellfish; Outbreaks; Norovirus; Environmental conditions. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12252/9031.pdf |
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| Registros recuperados: 67 | |
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